Description
Description: This assay employs a two-site sandwich ELISA to quantitate MPPE1 in samples. An antibody specific for MPPE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPPE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPPE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPPE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: MPPE1 gene contains 14 exons and spans about 27 kb. They detected 4 alternatively spliced forms: the most abundant form contains exons 1, 2, 5-12, and 14, and another form consists of exons 1, 2, and 5-14; the 2 rare forms correspond to these 2, but lack the sequences for exon 10.
The most abundant form encodes a deduced 396-amino acid protein with a signal sequence, a metallophosphoesterase domain, and a putative transmembrane domain. One of the alternative forms encodes a truncated 340-amino acid protein. Northern blot analysis detected a single 2.2-kb MPPE1 mRNA in brain, but not in any other tissues tested. The 3 alternative forms were detected in brain by RT-PCR only